Fig 2: TMP increases synapse-associated protein expression in BCCAO/CRS rats. (A–D) Representative immunoblots representing the expression and semi-quantitative analysis of synapse-associated proteins including PSD-95 (A), SYN (B), GAP-43 (C), and SYP (D). (E) Representative immunostaining showing the colocalization of MAP (green) and SYN (red) in the hippocampus of CA3 and DG. The inserts were high magnification from each group. Nuclei were counterstained with DAPI. Scale bar: Scale bar: 400 μm; and 200 μm for enlarged inserts. (F) Quantification of the fluorescence intensity of SYN in hippocampal CA3. (G) Bar graph summarizing the fluorescence intensity of SYN in hippocampal DG. **P < 0.01 vs. Sham. #P < 0.05, ##P < 0.01 vs. BCCAO/CRS. Data are presented as mean ± SE. n = 6 per group for Western blotting; n = 3 in each group and 3 slices per rat for immunofluorescence.

Fig 3: Identification of subgroups in SCLC tumorsINSM1/YAP1 mRNA expression ratios using data from the A. Rudin et al [14], B. George et al [15], and C. CCLE datasets [33]. Colored bars represent potential subgroup assignments in A. and B.. *Tumors with YAP1 mRNA = 0 in B. thus ratio cannot be calculated. D. INSM1 and YAP1 IHC on consecutive sections of four distinct tumor cores in discovery TMA, identified at top, each shown at two magnifications (shown to right). Slides were counterstained to show histology. Plots of INSM1 versus YAP1 E., SYP F. and ASCL1 G. IHC scores in discovery TMA using data from Supplementary Table S3. Size of circles proportional to number of cores represented. Blue circles represent cores with a high YAP1 score.